Isolation and characterization of the Shiga toxin gene (stx)-bearing Escherichia coli O157 and Non-O157 from retail meats in Shandong Province, CHINA, and characterization of the O157-derived stx2 phages - (Journal of Food Protection®, Volume 71, Number 4, April 2008 , pp. 706-713(8))
April 7, 2008 – 9:32 pmhttp://www.ingentaconnect.com/content/iafp/jfp/2008/00000071/00000004/art00005;jsessionid=qm32g3cqfe3p.victoria
Abstract:
Infection by Shiga toxin (Stx)-producing Escherichia coli of non-O157 and O157 serotypes are rare in China, but infection by O157 serotype was found in Shandong Province and three other provinces in China. To understand the reason for these rare infections and to determine the safety of retail meats in Shandong Province, we examined the distribution of Shiga toxin gene (stx)-bearing E. coli in retail meats and characterized the isolated stx-bearing strains.
We used hybridization with DNA probes and isolated stx1- and/or stx2-positive E. coli from 31 (58%) of 53 retail meat samples, with beef showing the highest frequency (68%). Of 42 stx-positive isolates, none belonged to O157. Using the O157-specific immunomagnetic bead technique, we isolated E. coli O157 carrying the eae and stx2 genes from eight beef samples (26%). These strains produced little or no Stx2 and carried a unique q gene. Replication of the stx2 phages was detected in […]
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