Impact of dilution ratios on Listeria monocytogenes growth during University of VERMONT medium enrichment of deli meats - (Journal of Food Protection®)
November 21, 2007 – 8:27 pmABSTRACT: In the U.S. Department of Agriculture (USDA) method for Listeria detection, a 25-g composite food sample is enriched in 225 ml of University of Vermont medium (UVM), giving a detection limit of 0.04 CFU/g.
However, in a recent large-scale four-state deli meat survey for L. monocytogenes, 125-g samples enriched in 1,125 ml of UVM were requested to increase the detection limit to 0.008 CFU/g. To circumvent problems associated with large volumes of UVM, the impact on L. monocytogenes growth of lower dilution ratios used for enrichment and most-probable-number (MPN) detection was compared with the results obtained using the conventional 1:10 dilution. In this study, 125-g samples of cured turkey, uncured turkey, ham, and roast beef were inoculated with a six-strain L.
monocytogenes cocktail to contain _1 _ 103 CFU/g. This cocktail was then diluted 1:3, 1:5, or 1:10 in UVM, homogenized, enriched at 30°C, and periodically plated on modified […]
MORE:
http://www.extension.iastate.edu/foodsafety/news/fsnews.cfm?newsid=22699
Related posts:
Resistance of Listeria monocytogenes F2365 cells to synthetic gastric fluid Is greater following growth on ready-to-eat deli turkey meat than in brain heart infusion broth - (Journal of Food Protection®)
ABSTRACT: Modeling transfer of Listeria monocytogenes from slicer to deli meat during mechanical slicing - (Journal of Foodborne Pathogens and Disease 5(2): 135-146)
Different enrichment procedures for recovery of Listeria monocytogenes from raw chicken samples can affect the results of detection (by chromogenic plating or real-time PCR) and lineage or strain identification - (Journal of Food Protection)
ABSTRACT: Inhibition of Listeria monocytogenes growth in cured ready-to-eat meat products by use of sodium benzoate and sodium diacetate - (Journal of Food Protection, Volume 71, Number 7, July 2008 , pp. 1386-1392(7))
Survival and growth of Listeria monocytogenes in broth as a function of temperature, pH, and potassium lactate and sodium diacetate concentrations - (Journal of Food Protection®, Volume 70, Number 11, November 2007 , pp. 2620-2625(6))
Growth of acid-adapted Listeria monocytogenes in orange juice and in minimally processed orange slices - (Food Control)
Effects of essential oils of oregano and nutmeg on growth and survival of Yersinia enterocolitica and Listeria monocytogenes in barbecued chicken - (Journal of Food Protection®, Volume 70, Number 11, November 2007 , pp. 2626-2630(5))
ABSTRACT: Protein expression by Listeria monocytogenes grown on a RTE-meat matrix - (International Journal of Food Microbiology Volume 128, Issue 2, 10 December 2008, Pages 203-211)
Survival of Listeria monocytogenes inoculated in retail soymilk products - (Journal of Food Control, Volume 19, Issue 9)
Inhibition of Listeria monocytogenes in cooked ham through active packaging with natural antimicrobials and high-pressure processing - (Journal of Food Protection®)
Corn-based film foils food-poisoning bugs - (New Scientist)
GEORGIA: Effect of electron beam irradiation on the safety of diced chicken meat and turkey frankfurters - (University of Georgia)
ABSTRACT: Antibacterial efficiency of Spanish Satureja montana essential oil against Listeria monocytogenes among natural flora in minced pork - (Journal of Food Protection)
CANADA: Health hazard alert Oven roasted turkey breast sold by White House Meats Inc., Toronto may contain Listeria - (Canadian Food Inspection Agency)
Inactivation of Listeria innocua on frankfurters that contain potassium lactate and sodium diacetate by flash pasteurization - (Journal of Food Science (OnlineEarly Articles). doi:10.1111/j.1750-3841.2007.00635.x)
UK: Reminder on safe storage of freshly cooked sliced meats - (Medical News)
US: EPA approved antimicrobial could control listeria in food plants - (Food Production Daily)
ITALY: Evaluation of a rapid molecular method for detection of Listeria monocytogenes directly from enrichment broth media - (Food Control)
Antimicrobial efficacy of eugenol microemulsions in milk against Listeria monocytogenes and Escherichia coli O157:H7 - (Journal of Food Protection®, Volume 70, Number 11, November 2007 , pp. 2631-2637(7))
BARFBLOG: Surprise: Research finds Canadians consumed fewer deli-meats after listeria killed 20 - (barfblog)
BARFBLOG: Michael McCain of Maple Leaf: Should pregnant women eat deli meats? - (barfblog)
Listeria prevalence and Listeria monocytogenes serovar diversity at cull cow and bull processing plants in the UNITED STATES - (Journal of Food Protection®)
Free web seminars on controlling Listeria monocytogenes on ready-to-eat meat and poultry products and in the RTE processing environment - (University of Nebraska - Lincoln)
ABSTRACT: Modifications in membrane fatty acid composition of Salmonella typhimurium in response to growth conditions and their effect on heat resistance - (Journal of Food Microbiology, Volume 123, Issue 3)
Health hazard alert Roast beef sold at certain deli counters in ONTARIO may contain Listeria monocytogenes - (CFIA)
ABSTRACT: Individual effects of sodium, potassium, calcium, and magnesium chloride salts on Lactobacillus pentosus and Saccharomyces cerevisiae growth - (Journal of Food Protection, Volume 71, Number 7, July 2008 , pp. 1412-1421(10))
Elimination of Listeria monocytogenes on hotdogs by infrared surface treatment - (Journal of Food Science)
ABSTRACT: The poloxamer P85 is protective against Listeria monocytogenes invasion - (Foodborne Pathogens and Disease, 5(6): 859-865)
ABSTRACT: Microbial changes and growth of Listeria monocytogenes during chilled storage of brined shrimp (Pandalus borealis) - (International Journal of Food Microbiology Volume 124, Issue 3)
Monitoring hygiene on- and at-line is critical for controlling Listeria monocytogenes during produce processing - (Journal of Food Protection®, Volume 71, Number 4, April 2008 , pp. 735-741(7))
Photoactivated chlorophyllin-based gelatin films and coatings to prevent microbial contamination of food products - (International Journal of Food Microbiolgy (Volume 126, Issues 1-2, Pages 1-262))
Effect of combining nisin and/or lysozyme with in-package pasteurization for control of Listeria monocytogenes in ready-to-eat turkey bologna during refrigerated storage - (Food Microbiology Volume 25, Issue 7)
US: Elimination of listeria monocytogenes on hotdogs by infrared - (Journal of Food Science)
ABSTRACT: Role of sigma B factor in the alkaline tolerance response of Listeria monocytogenes 10403S and cross-protection against subsequent ethanol and osmotic stress - (Journal of Food Protection, Volume 71, Number 7, July 2008 , pp. 1481-1485(5))
FDA: Control of Listeria monocytogenes in refrigerated or frozen ready-to-eat foods - (Guidance for Industry)
Listeria a nightmare in a processing plant - (Globe and Mail)
Bacteriophage-based enrichment coupled to Immunochromatographic strip-based detection for the determination of Salmonella in meat and poultry - (Journal of Food Protection®, Volume 70, Number 10, October 2007 , pp. 2235-2242(8))
BARFBLOG: Should deli meats be on the menu for pregnant women and at medical care facilities? - (barfblog)
ONTARIO: Health Hazard Alert - Certain ready-to-eat deli meat products produced at establishment 97B may contain Listeria monocytogenes - (Canadian Food Inspection Agency)
CANADA: Hospitals, care facilities ignored listeria warnings for three years - (The Province)
Control of Listeria monocytogenes and Salmonella anatum on the surface of smoked salmon coated with calcium alginate coating containing oyster lysozyme and nisin - (Journal of Food Science, Volume 73 Issue 2 Page M67-M71, March 2008)
ABSTRACT: Microbial growth and the effects of mild acidification and preservatives in refrigerated sweet potato puree - (Journal of Food Protection)
Inactivation kinetics of inoculated Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Poona on whole cantaloupe by chlorine dioxide gasstar, open - (Food Microbiology Volume 25, Issue 7)
Growth response of Salmonella typhimurium in the presence of natural and synthetic antimicrobials: Estimation of MICs from three different models - (Journal of Food Protection®, Volume 70, Number 10, October 2007 , pp. 2243-2250(8))
Kraft patents new method for detecting listeria - (Food Innovation Weekly)
CANADA: Health Hazard Alert Shopsys deli-fresh Classic Reuben sandwich may contain Listeria monocytogenes - (Canadian Food Inspection Agency)
CANADA: Consumers changed habits following listeriosis outbreak, study finds - (University of Guelph)
Microbiological safety of sandwiches from hospitals and other health care establishments in the United Kingdom with a focus on Listeria monocytogenes and other Listeria species - (Journal of Food Protection)
Effect of biofilm dryness on the transfer of Listeria monocytogenes biofilms grown on stainless steel to bologna and hard salami - (Journal of Food Protection®)
ONTARIO: Health Hazard Alert - Shopsy’s Deli-Fresh Classic Reuben sandwich may contain Listeria monocytogenes - (Canadian Food Inspection Agency)
ABSTRACT: Inactivation of Salmonella, E. coli and Listeria monocytogenes in phosphate-buffered saline and apple juice by ultraviolet and heat treatments - (Food Control (Volume 20, Issue 4, Pages 443-446))
ABSTRACT: Edible coatings for enhancing microbial safety and extending shelf life of hard-boiled eggs - (Journal of Food Science)
UK: Health experts warn of listeria dangers in meat - (Liverpool Daily Post)
Antimicrobial activity of Lactoferrin against foodborne pathogenic bacteria incorporated into edible Chitosan film - (Journal of Food Protection)
Adaptive response of Listeria monocytogenes to heat and its impact on food safety - (Food Control)
Antimicrobial effect of electrolyzed oxidizing water against Escherichia coli O157:H7 and Listeria monocytogenes on fresh strawberries - (Journal of Food Science)
BARFBLOG: Maple Leaf says listeria happens; Carl says, stop whining - (barfblog)
ONTARIO: Health Canada approves use of preservative that could stem growth of Listeria - (Canadian Press)
COLORADO state scientists work to control Listeria outbreak - (Colorado State University)
BARFBLOG: 17 confirmed and suspected dead in Canadian listeria outbreak - (barfblog)
CANADA: CFIA to launch new listeria testing protocols - (CBC.ca News)
CANADA: CFIA to launch new listeria testing protocols - (CBC.ca News)
Listeria monocytogenes: The survivor amongst bacteria - (Newsfood.com)
Survival of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in cranberry juice concentrates at different °Brix levels - (Journal of Food Protection (Volume 70, Number 9, pp. 2072-2077))
ONTARIO: Health Hazard Alert - Old Style pastrami may contain Listeria monocytogenes - (Canadian Food Inspection Agency)
ABSTRACT: Potential antimicrobials to control Listeria monocytogenes in vacuum-packaged cold-smoked salmon pâté and fillets - (Journal of Food Microbiology, Volume 123, Issue 3)
ABSTRACT: Effect of Inoculum preparation procedure and storage time and temperature on the fate of Listeria monocytogenes on inoculated salami - (Journal of Food Protection)
Interactions in dual species biofilms between Listeria monocytogenes EGD-e and several strains of Staphylococcus aureus - (International Journal of Food Microbiolgy (Volume 126, Issues 1-2, Pages 1-262))
CANADA: Health hazard alert - Old Style brand smoked pork loin chops and thin sliced bacon may contain listeria monocytogenes - (CFIA)
More warnings needed about listeriosis dangers, expert says - (Canwest News Service)
ABSTRACT: Use of UV light for the inactivation of Listeria monocytogenes and lactic acid bacteria species in recirculated chill brines - (Journal of Food Protection)
UK: Continental sausages under the spotlight - (The Argus Lite)
ABSTRACT: Occurrence of listeria and enterobacteriaceae in domestic refrigerators - (Journal of Food Protection)
ABSTRACT: Processing plant persistent strains of Listeria monocytogenes appear to have a lower virulence potential than clinical strains in selected virulence models - (Journal of Food Microbiology, Volume 123, Issue 3)
ONTARIO: Another death tied to tainted meat - (Winnipeg Sun)
Preliminary analysis of the lipase gene (gehM) expression of Staphylococcus xylosus in vitro and during fermentation of naturally fermented sausages (in situ) - (Journal of Food Protection®)
ONTARIO: Updated Health Hazard Alert - Deli roast beef and sandwiches sold at certain stores in Atlantic Canada may contain Listeria monocytogenes - (Canadian Food Inspection Agency)
Evaluation of the effect of acetylsalicylic acid on Clostridium botulinum growth and toxin production - (Journal of Food Protection)
TORONTO: Education the answer to raw-milk cheese flap - (The Toronto Star)
Slim chance of listeria cross-contamination - (CJAD)
UK: Low levels of listeria in smoked fish - (Food Standards Agency (UK))
Listeria monocytogenes and Escherichia coli O157:H7 inhibition in vitro by liposome-encapsulated nisin and ethylene diaminetetraacetic acid - (Journal of Food Safety Vol. 28, Issue 2)
BARFBLOG: Seven deaths, dozens of illness investigated in Canadian listeria outbreak: pregnant women reminded of risks - (barfblog)
CALIFORNIA: Listeria control workshop for small business - (Dept. Food Science and Technology, University of Nebraska - Lincoln)
Prevalence of Escherichia coli O157:H7 in the AMERICAN bison (Bison bison) - (Journal of Food Protection®)
U.S.: Listeria control in consumer protein products - (Institute of Food Technologists)
MISSOURI: Red wine protects against food-borne germs - (Jamaica Gleaner)
CANADA: Listeriosis outbreak heightens food scrutiny - (The Globe and Mail)
ARKANSAS: Researchers use edible whey coatings to fight pathogens - (Meatingplace.com)
AUSTRALIA: Food Recall Alert - (FSANZ)
SPROUTNET: Garbage in garbage out - (International Specialty Supply)
BARFBLOG: Canadian consumers, if you have Maple Leaf deli meats, its your fault - (barfblog)
ARIZONA: Illness concerns at Tucson deli - roach concerns at Tucson bistro - (News 4)
The claim: Mayonnaise can increase the risk of food poisoning - (New York Times)
ABSTRACT: Influence of selective media on successful detection of Shiga toxinproducing Escherichia coli in food, fecal, and environmental samples - (Foodborne Pathogens and Disease Volume: 5, Number: 3 Jun 2008)
Determination of 5-Log reduction times for food pathogens in acidified cucumbers during storage at 10 and 25°C - (Journal of Food Protection®, Volume 70, Number 11, November 2007 , pp. 2638-2641(4))
DENMARK: Morbidity and mortality of elderly patients with zoonotic Salmonella and Campylobacter: A population-based study - (Journal of Infection, Volume 57, Issue 3, pg. 214-222)
PENNSYLVANIA: Washing mushrooms stalls germs, extends shelf life - (Penn State Live)
VERMONT: UVM illness may be linked to dining hall - (Burlington Free Press)
Risk profiles of pork and poultry meat and risk ratings of various pathogen/product combinations - (International Journal of Food Microbiolgy (Volume 126, Issues 1-2, Pages 1-262))