Effect of biofilm dryness on the transfer of Listeria monocytogenes biofilms grown on stainless steel to bologna and hard salami - (Journal of Food Protection®)
November 21, 2007 – 7:59 pmABSTRACT: Listeria monocytogenes continues to be a major cause of class I food recalls in the United States. Very little is known about its transfer and cross-contamination in processing scenarios. The objective of this study was to evaluate the effect of hydration level on L. monocytogenes biofilms grown on stainless steel and its effect on the biofilm transfer to foods. Biofilms were grown on stainless steel in diluted tryptic soy broth 1:20 for 48 h at 32°C.
After this, biofilms were equilibrated over saturated salt solutions at 20°C for 24 h (94, 75, 58, and 33% relative humidity; % RH) prior to transferring.
Transfer experiments were conducted from inoculated stainless steel to bologna and hard salami at a constant pressure (45 kPa) and time (30 s) with a universal testing machine. The experiment was designed with a factorial design 4 _ 2 (biofilms equilibrated at 4% RH and two foods) […]
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